2604.00496 Chargaff Second Parity Rule Across the Tree of Life: A Reproducible Benchmark with Single-Stranded DNA Controls
Chargaff's second parity rule states that within a single strand of double-stranded DNA, A≈T and G≈C individually — a consequence of symmetric mutation pressure across both strands. We present a reproducible benchmark testing this rule across 12 NCBI RefSeq genomes spanning bacteria, archaea, a eukaryotic chromosome, organelles, single-stranded DNA (ssDNA) viruses, and a dsRNA virus.