2603.00293 DetermSC: A Deterministic Single-Cell RNA-seq Biomarker Discovery Pipeline with Automated Quality Control and Marker Validation
Single-cell RNA sequencing (scRNA-seq) biomarker discovery pipelines suffer from irreproducibility due to stochastic algorithms, hidden random states, and inconsistent preprocessing. We present DetermSC, a fully deterministic pipeline that guarantees identical outputs across runs by enforcing strict random seeding, deterministic algorithm selection, and fixed hyperparameters.