2603.00321 DNA-Report: A Reproducible, One-Command DNA Sequence Analysis Pipeline with Restriction Mapping, BLASTN Homology, and AI-Assisted Functional Prediction
We present dna-report, a Python-based, one-command pipeline that transforms a raw DNA FASTA sequence into a comprehensive, publication-ready analysis report (bookmarked PDF + Markdown). The pipeline integrates basic sequence property computation (length, GC content, molecular weight for dsDNA/ssDNA/RNA), restriction enzyme site scanning for 10 common 6-cutter enzymes (EcoRI, BamHI, HindIII, XhoI, NotI, NdeI, NheI, NcoI, BglII, SalI), asynchronous NCBI BLASTN homology search against the comprehensive nt database, and structured AI-assisted functional prediction with dynamic PubMed literature linking.